cFluor<sup>®</sup> BYG750 Anti-Human CD5 (L17F12)
The L17F12 monoclonal antibody binds to human CD5, a 67-kDa single chain type I glycoprotein in the scavenger receptor family(1)(2). CD5, also known as Leu-1, Ly-1 or T1, is expressed on thymocytes, T cells and subsets of B cells. CD5 binds to its ligand on B cells, CD72 (LYB-2), to play a role in T-B cell interaction and to modulate the intensity of antigen receptor signal transduction(3). The antibody was conjugated to a fluorophore and purified by affinity chromatography.
PRODUCT DETAILS
Clone: L17F12
Reactivity: Human
Format: cFluor® BYG750*
Isotype: Mouse IgG2a, κ
Tested Dilution: 5 μL / test
Application: Flow Cytometry
Storage: 2-8°C and protected from light. Do not freeze
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)
RECOMMENDED USAGE
Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.
Please briefly centrifuge the reagent vial before use.
Use appropriate personal protective equipment per the product safety data sheet when using this product.
REFERENCES
- Engleman EG, et al. 1981. Proc Natl Acad Sci USA. 78(3):1791-5
- Velasco-de Andrés M, et al. 2020. Cells vol. 9,12 2589
- Tabbekh M, et al. 2013. Oncoimmunology 2,1
*cFluor® BYG750 is a tandem dye made with R-PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.
For Research Use Only. Not intended for use in diagnostic procedures.
The L17F12 monoclonal antibody binds to human CD5, a 67-kDa single chain type I glycoprotein in the scavenger receptor family(1)(2). CD5, also known as Leu-1, Ly-1 or T1, is expressed on thymocytes, T cells and subsets of B cells. CD5 binds to its ligand on B cells, CD72 (LYB-2), to play a role in T-B cell interaction and to modulate the intensity of antigen receptor signal transduction(3). The antibody was conjugated to a fluorophore and purified by affinity chromatography.
PRODUCT DETAILS
Clone: L17F12
Reactivity: Human
Format: cFluor® BYG750*
Isotype: Mouse IgG2a, κ
Tested Dilution: 5 μL / test
Application: Flow Cytometry
Storage: 2-8°C and protected from light. Do not freeze
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)
RECOMMENDED USAGE
Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.
Please briefly centrifuge the reagent vial before use.
Use appropriate personal protective equipment per the product safety data sheet when using this product.
REFERENCES
- Engleman EG, et al. 1981. Proc Natl Acad Sci USA. 78(3):1791-5
- Velasco-de Andrés M, et al. 2020. Cells vol. 9,12 2589
- Tabbekh M, et al. 2013. Oncoimmunology 2,1
*cFluor® BYG750 is a tandem dye made with R-PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.
For Research Use Only. Not intended for use in diagnostic procedures.
Original: $222.00
-70%$222.00
$66.60Description
The L17F12 monoclonal antibody binds to human CD5, a 67-kDa single chain type I glycoprotein in the scavenger receptor family(1)(2). CD5, also known as Leu-1, Ly-1 or T1, is expressed on thymocytes, T cells and subsets of B cells. CD5 binds to its ligand on B cells, CD72 (LYB-2), to play a role in T-B cell interaction and to modulate the intensity of antigen receptor signal transduction(3). The antibody was conjugated to a fluorophore and purified by affinity chromatography.
PRODUCT DETAILS
Clone: L17F12
Reactivity: Human
Format: cFluor® BYG750*
Isotype: Mouse IgG2a, κ
Tested Dilution: 5 μL / test
Application: Flow Cytometry
Storage: 2-8°C and protected from light. Do not freeze
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)
RECOMMENDED USAGE
Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.
Please briefly centrifuge the reagent vial before use.
Use appropriate personal protective equipment per the product safety data sheet when using this product.
REFERENCES
- Engleman EG, et al. 1981. Proc Natl Acad Sci USA. 78(3):1791-5
- Velasco-de Andrés M, et al. 2020. Cells vol. 9,12 2589
- Tabbekh M, et al. 2013. Oncoimmunology 2,1
*cFluor® BYG750 is a tandem dye made with R-PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.
For Research Use Only. Not intended for use in diagnostic procedures.
